A Novel Clotting Assay for Quantitation of Plasma Prothrombin (Factor II) UsingEchis multisquamatusVenom
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Platelet Factor 3 Based-clotting Time Assay as a Quality Marker for Long-term Storage of Platelet Concentrates
Background: Platelets rapidly lose their qualities usually after 5 day of storage. Different standard methods have been recommended to check the quality of platelets during storage which some of them show better correlation with other quality markers during storage. The purpose of this study was to demonstrate if platelet factor 3 (PF3) assay could be an indicator of storage lesion and provide ...
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References 1. Sunderman FW. The clinical biochemistry of 59-nucleotidase [Review]. Ann Clin Lab Sci 1990;20:123–39. 2. Bodansky O, Schwartz MK. 59-Nucleotidase [Review]. Adv Clin Chem 1968; 11:277–328. 3. Dixon TF, Purdom M. Serum 59-nucleotidase. J Clin Pathol 1954;7:341–3. 4. Eshchar J, Rudzki C, Zimmerman HJ. Serum levels of 59-nucleotidase in disease. Am J Clin Pathol 1967;47:598–606. 5. Be...
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Recent study of 400 human cases, including 240 with diverse hemorrhagic disorders, has accumulated noteworthy experience with a wide variety of test methods for quantitating the collective and individual factors in the blood clotting and hemostatic mechanisms. Important among these tests are those dealing with prothrombin and its conversion to thrombin for the enzymatic polymerization of fibrin...
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A coupled amidolytic assay for factor VII (VII) has been developed that when used with a clotting assay for VII enables detection of activated VII. In the assay, VII in a test material determines generation of factor Xa in a mixture of purified factor X, tissue factor, and calcium; factor Xa is measured with a chromogenic substrate. Factor VII activity in the coupled amidolytic assay (VIIam) co...
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We have investigated the pathway of prothrombin activation in blood and plasma. By means of a rapid purification procedure involving chromatography on DEAE-cellulose and hydroxyapatite, we demonstrated that the major prothrombin fragment in serum is that representing the amino-terminal half of prothrombin (i.e. F1-2). The F1-2 isolated was characterized by its size, amino acid and antigenic com...
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ژورنال
عنوان ژورنال: American Journal of Clinical Pathology
سال: 1999
ISSN: 0002-9173,1943-7722
DOI: 10.1093/ajcp/112.5.705